Composite

Part:BBa_K1677000

Designed by: Isabelle Capell-Hattam   Group: iGEM15_BABS_UNSW_Australia   (2015-07-15)


IPTG Inducible Tsi2 Antitoxin

This is an IPTG Inducible promoter (with RBS) that produces the Tsi2 Antitoxin. This part, when expressed on a different plasmid to Tse2 (under a constitutive promoter), can prevent horizontal gene transfer of the Tse2 plasmid and can will also prevent escape of the GMO bacteria from outside of the lab (where IPTG isn't present)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 448
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 443


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Categories
Parameters
None